Livin Regulates H2A.XY142 Phosphorylation and Promotes Autophagy in Colon Cancer Cells via a Novel Kinase Activity
Identifieur interne : 000686 ( Main/Exploration ); précédent : 000685; suivant : 000687Livin Regulates H2A.XY142 Phosphorylation and Promotes Autophagy in Colon Cancer Cells via a Novel Kinase Activity
Auteurs : Yang Ge ; Bao-Lin Liu ; Jun-Peng Cui ; Shu-Qiang LiSource :
- Frontiers in Oncology [ 2234-943X ] ; 2019.
Abstract
Url:
DOI: 10.3389/fonc.2019.01233
PubMed: 31799193
PubMed Central: 6868062
Affiliations:
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Livin Regulates H2A.X<sup>Y142</sup> Phosphorylation and Promotes Autophagy in Colon Cancer Cells via a Novel Kinase Activity</title><author><name sortKey="Ge, Yang" sort="Ge, Yang" uniqKey="Ge Y" first="Yang" last="Ge">Yang Ge</name></author><author><name sortKey="Liu, Bao Lin" sort="Liu, Bao Lin" uniqKey="Liu B" first="Bao-Lin" last="Liu">Bao-Lin Liu</name></author><author><name sortKey="Cui, Jun Peng" sort="Cui, Jun Peng" uniqKey="Cui J" first="Jun-Peng" last="Cui">Jun-Peng Cui</name></author><author><name sortKey="Li, Shu Qiang" sort="Li, Shu Qiang" uniqKey="Li S" first="Shu-Qiang" last="Li">Shu-Qiang Li</name></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">31799193</idno><idno type="pmc">6868062</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6868062</idno><idno type="RBID">PMC:6868062</idno><idno type="doi">10.3389/fonc.2019.01233</idno><date when="2019">2019</date><idno type="wicri:Area/Pmc/Corpus">000565</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000565</idno><idno type="wicri:Area/Pmc/Curation">000565</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000565</idno><idno type="wicri:Area/Pmc/Checkpoint">000634</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000634</idno><idno type="wicri:Area/Ncbi/Merge">000C76</idno><idno type="wicri:Area/Ncbi/Curation">000C76</idno><idno type="wicri:Area/Ncbi/Checkpoint">000C76</idno><idno type="wicri:Area/Main/Merge">000686</idno><idno type="wicri:Area/Main/Curation">000686</idno><idno type="wicri:Area/Main/Exploration">000686</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Livin Regulates H2A.X<sup>Y142</sup> Phosphorylation and Promotes Autophagy in Colon Cancer Cells via a Novel Kinase Activity</title><author><name sortKey="Ge, Yang" sort="Ge, Yang" uniqKey="Ge Y" first="Yang" last="Ge">Yang Ge</name></author><author><name sortKey="Liu, Bao Lin" sort="Liu, Bao Lin" uniqKey="Liu B" first="Bao-Lin" last="Liu">Bao-Lin Liu</name></author><author><name sortKey="Cui, Jun Peng" sort="Cui, Jun Peng" uniqKey="Cui J" first="Jun-Peng" last="Cui">Jun-Peng Cui</name></author><author><name sortKey="Li, Shu Qiang" sort="Li, Shu Qiang" uniqKey="Li S" first="Shu-Qiang" last="Li">Shu-Qiang Li</name></author></analytic><series><title level="j">Frontiers in Oncology</title><idno type="eISSN">2234-943X</idno><imprint><date when="2019">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p><bold>Objective:</bold> To investigate Livin-mediated regulation of H2A.X<sup>Y142</sup> phosphorylation via a novel kinase activity and its effect on autophagy in colon cancer cells.</p><p><bold>Methods:</bold> The interaction between Livin and H2A.X was tested by immunoprecipitation. H2A.X–/– HCT116 cells were transfected with human influenza hemagglutinin (HA)-tagged WT or Y142F phospho-dead mutantH2A.X plasmids. GST-tagged recombinant Livin protein was used to perform <italic>in vitro</italic> pull-down experiment and kinase assay. H2A.X–/–Livin+/+ SW480 cells were co-transfected with H2A.X<sup>WT</sup>/H2A.X<sup>Y142F</sup> plasmid and LC3 EGFP-tagged plasmid to explore whether H2A.X<sup>Y142F</sup> was involved in Livin-mediated autophagy induced by starvation in colon cancer cells.</p><p><bold>Results:</bold> Co-immunoprecipitation studies confirmed that Livin interacted with H2A.X and that it was phosphorylation dependent. <italic>In vitro</italic> kinase assay confirmed that Livin could phosphorylate H2A.X. Knockdown of Livin (Livin–/–) in SW480 cells or HCT116 cells canceled the starvation-induced autophagy in colon cancer cells; H2A.X–/–Livin+/+ SW480 cells transfected with H2A.X<sup>WT</sup> activated autophagy induced by starvation while cells transfected with H2A.X<sup>Y142F</sup> had no significant difference; Livin-H2A.X<sup>Y142F</sup> axis activated autophagy in colon cancer cells through transcriptionally regulating <italic>ATG5</italic> and <italic>ATG7</italic>.</p><p><bold>Conclusion:</bold> Livin promotes autophagy in colon cancer cells via regulating the phosphorylation of H2A.X<sup>Y142</sup>.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Castillo, J" uniqKey="Castillo J">J Castillo</name></author><author><name sortKey="Lopez Rodas, G" uniqKey="Lopez Rodas G">G Lopez-Rodas</name></author><author><name sortKey="Franco, L" uniqKey="Franco L">L 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uniqKey="Polo S">SE Polo</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lorkovic, Zj" uniqKey="Lorkovic Z">ZJ Lorkovic</name></author><author><name sortKey="Park, C" uniqKey="Park C">C Park</name></author><author><name sortKey="Goiser, M" uniqKey="Goiser M">M Goiser</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kumar, D" uniqKey="Kumar D">D Kumar</name></author><author><name sortKey="Tewari Singh, N" uniqKey="Tewari Singh N">N Tewari-Singh</name></author><author><name sortKey="Agarwal, C" uniqKey="Agarwal C">C Agarwal</name></author><author><name sortKey="Jain, Ak" uniqKey="Jain A">AK Jain</name></author><author><name sortKey="Inturi, S" uniqKey="Inturi S">S Inturi</name></author><author><name sortKey="Kant, R" uniqKey="Kant R">R Kant</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Beach, A" uniqKey="Beach A">A Beach</name></author><author><name sortKey="Groves, Am" 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